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levG [2018-07-26 14:52:31]
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levG [2018-07-26 14:52:31]

fructose-specific phosphotransferase system, EIID component of the PTS
locus
BSU27040
pI
9.94
mw
29.94 kDa
protein length
275 aa Sequence Blast
gene length
825 bp Sequence Blast
function
fructose uptake and phosphorylation
product
fructose-specific phosphotransferase systemEIID component
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.7|Utilization of fructose]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,760,233 → 2,761,060

    The protein

    Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, mannose permease (Man) family [Pubmed|10627040]

    • Expression and Regulation

    Operons

    genes
    [gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]
    description
    [Pubmed|2117666]

    sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|1924373], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7592486], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|1D2043CC0D8CF64142F0A5993A936C5A196726D4|LevR]: activation, [Pubmed|1900939], in [regulon|1D2043CC0D8CF64142F0A5993A936C5A196726D4|LevR regulon]

    • regulation

  • induced in the presence of fructose ([protein|search|LevR]) [Pubmed|1900939]
    • view in new tab

    Biological materials

    Mutant

  • BKE27040 (Δ[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCTTCCCCCTCAT, downstream forward: _UP4_TAAGGTGTGTGAAGGAAAGA
  • BKK27040 (Δ[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCTTCCCCCTCAT, downstream forward: _UP4_TAAGGTGTGTGAAGGAAAGA

    • References

  • 10627040,2117666,1924373,7592486,1900939